cAMP ELISA Kit (GXEL00385)

cAMP ELISA Kit

The cAMP ELISA Kit (SKU: GXEL00385) is a high-sensitivity quantitative ELISA assay developed for the quantitative measurement of cAMP in Plasma, Serum. The assay delivers a detection range of 1.56-200 pmol/mL and a sensitivity of 1.56 pmol/mL and is read colorimetrically at 450 nm, making it well suited to quantitative research workflows.

<p>Adenosine 3&#39;, 5&#39;-cyclic monophosphate (cAMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of G protein&ndash;coupled receptors(GPCRs). Bioyeargene cAMP ELISA Kit is based on the competition between HRP-labeled cAMP and free cAMP for fixed amounts of mouse monoclonal anti-cAMP antibody(cAMP MAb) binding sites. Because the concentration of the HRP-labeled cAMP is held constant while the concentration of cAMP varies, the amount of HRP-labeled cAMP that is able to bind to the cAMP MAb will be inversely proportional to the concentration of cAMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50(50% B/B0) of approximately 12 pmol/mL and a detection limit of approximately 0.15 pmol/mL.</p>

Supplied with pre-coated microplates and all core reagents, the cAMP ELISA Kit gives you a validated, ready-to-use solution for cAMP research. Get accurate and reliable results with the cAMP ELISA Kit from Assay Genie.