Autophagy in Neurodegeneration Antibody Sampler Pack

Autophagy in Neurodegeneration Antibody Sampler Pack

The Autophagy in Neurodegeneration Antibody Sampler Pack from Assay Genie brings together 8 rigorously validated antibodies spanning the key nodes of this pathway, supplied in a convenient 20µL trial size. It lets researchers screen an entire signalling axis in a single, cost-effective pack — validated for applications including ELISA, IF/ICC, IHC-P, IP, WB — before committing to full-size vials. Each antibody is also available individually in a full 100µL size.

Autophagy is the principal pathway by which neurons degrade and recycle damaged organelles and aggregation-prone proteins, and because neurons are post-mitotic and cannot dilute toxic species through division, they are exquisitely dependent on efficient autophagic clearance. Failure of this system is a recurrent theme across neurodegenerative disease, where accumulating misfolded proteins overwhelm or impair the degradation machinery. The formation of autophagosomes is initiated and nucleated by Beclin-1, a core component of the class III PI3K complex, and elongation of the autophagosomal membrane requires the conjugation systems built around ATG5 and ATG7, which together drive lipidation of LC3. LC3B is the canonical autophagosome marker; its conversion from the cytosolic form to the membrane-bound, lipidated form tracks autophagosome formation and flux. Cargo is delivered selectively by autophagy receptors that recognize ubiquitinated substrates. p62/SQSTM1 binds both Ubiquitin and LC3 to shuttle protein aggregates into autophagosomes, and its accumulation signals impaired flux, while Optineurin is a related receptor important for the clearance of damaged mitochondria and protein aggregates, with mutations linked to amyotrophic lateral sclerosis and glaucoma. Ubiquitin itself tags substrates for selective degradation and is a constituent of the inclusions found in many neurodegenerative conditions. The entire system is transcriptionally coordinated by TFEB, a master regulator that, upon activation, translocates to the nucleus to induce autophagy and lysosomal biogenesis genes, offering a potential therapeutic lever to enhance clearance. Together these proteins define the initiation, elongation, cargo selection, and transcriptional control of neuronal autophagy. This pack brings together validated antibodies against LC3B, p62/SQSTM1, Beclin-1, ATG5, ATG7, Ubiquitin, TFEB, and Optineurin to support investigation of autophagic dysfunction in neurodegeneration.