The VAMP8 Monoclonal Antibody (CAB4728) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for use in Western blot applications. By binding to the VAMP8 protein, this antibody enables the detection and analysis of VAMP8 in various cell types, facilitating investigations in cell biology and neurobiology.VAMP8, also known as vesicle-associated membrane protein 8, plays a crucial role in regulating the secretion of various molecules, including neurotransmitters and immune mediators.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
VAMP8 Monoclonal Antibody
SKU:
CAB4728
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1108
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EDB, VAMP-8, VAMP8
Positive Sample:
HeLa, K-562, Mouse liver, Mouse kidney, Rat lung
Cellular Localization:
Cytoplasm, Cytosol, Early Endosome, Extracellular Exosome, Perinuclear Region Of Cytoplasm, Plasma Membrane, Recycling Endosome, Snare Complex.
Calculated MW:
11kDa
Observed MW:
15kDa
This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane.
Purification Method
Affinity purification
Gene ID
8673
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using VAMP8 Rabbit mAb (CAB4728) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of various lysates using VAMP8 Rabbit mAb (CAB4728) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
