The HTF9C Monoclonal Antibody (CAB0136) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications.TRMT2A, also known as HTF9C, plays a crucial role in modulating gene expression and protein synthesis by regulating tRNA modifications. Dysregulation of TRMT2A has been implicated in various diseases, including cancer and developmental disorders, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HTF9C Monoclonal Antibody
SKU:
CAB0136
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2498
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HTF9C, TRMT2A/HTF9C
Positive Sample:
MCF7, K-562
Cellular Localization:
Cytosol.
Calculated MW:
69kDa
Observed MW:
75kDa
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
27037
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis lysates from K-562 cells using TRMT2A/HTF9C Rabbit mAb (CAB0136) at 1:1000 dilution incubated overnight at 4°C Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1 s.
Western blot analysis lysates from MCF7 cells using TRMT2A/HTF9C Rabbit mAb (CAB0136) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
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