The SP7 Antibody (CAB18699) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human, mouse, and rat samples, making it suitable for a wide range of research applications.Sp7, also known as Osterix, plays a crucial role in regulating the differentiation of mesenchymal stem cells into osteoblasts, the cells responsible for bone formation. Understanding the role of Sp7 in bone development and remodeling is essential for developing new therapies for bone-related diseases such as osteoporosis and osteogenesis imperfecta.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SP7 Antibody
SKU:
CAB18699
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OSX, OI11, OI12, osterix, SP7
Positive Sample:
U2OS, Mouse bone, Rat brain
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
45kDa
Observed MW:
45kDa/46kDa
This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.
Purification Method
Affinity purification
Gene ID
121340
RRID
AB_2862433
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from U2OS cells, using SP7 Rabbit pAb (CAB18699) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Western blot analysis of lysates from Mouse bone, using SP7 Rabbit pAb (CAB18699) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
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