Description
SARS-CoV-2 NSP9 Antibody (CAB20308)
The SARS-CoV-2 NSP9 Antibody (CAB20308) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is specifically designed to target the NSP9 protein of the virus, which plays a key role in viral replication and transcription processes. By binding to NSP9, researchers can detect and analyze the protein in various samples, allowing for a better understanding of the virus and potential therapeutic targets.Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against SARS-CoV-2 samples.
| Product Name: | SARS-CoV-2 NSP9 Antibody |
| SKU: | CAB20308 |
| Size: | 20μL, 100μL |
| Reactivity: | SARS-CoV-2 |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | NNEL SPVA LRQM SCAA GTTQ TACT DDNA LAYY NTTK GGRF VLAL LSDL QDLK WARF PKSD GTGT IYTE LEPP CRFV TDTP KGPK VKYL YFIK GLNN LNRG MVLG SLAA TVRL Q | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
|
| Positive Sample: | 293T |
| Calculated MW: | 794kDa |
| Observed MW: | 15-20kDa |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.
| Purification Method | Affinity purification |
| Gene ID | 43740578 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of extracts of normal 293T cells and 293T transfected with NSP9 Protein, using SARS-CoV-2 NSP9 Rabbit pAb (CAB20308) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s. |
