The NEK7 Polyclonal Antibody (CAB21088) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for use in various applications, including Western blotting.NEK7, a member of the NIMA-related kinase family, plays a crucial role in mitotic spindle assembly and chromosome segregation during cell division. Dysregulation of NEK7 has been linked to aberrant cell proliferation and tumorigenesis, highlighting its importance in cancer biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NEK7 Polyclonal Antibody
SKU:
CAB21088
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Jurkat, HepG2, Mouse lung, Rat liver
Cellular Localization:
Cytoplasm, Microtubule Organizing Center, Nucleoplasm, Nucleus, Spindle Pole.
Calculated MW:
35kDa
Observed MW:
32kDa
NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.
Purification Method
Affinity purification
Gene ID
140609
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NEK7 Rabbit pAb (CAB21088) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of various lysates using NEK7 Rabbit pAb (CAB21088) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
