Anti-HDEL Antibody [2E7]

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SKU:
STMA00046
SKU:
STMA00046
Antibody Type:
Monoclonal
Applications:
WB
Applications:
ICC/IF
Reactivity:
Fruit Fly (Drosophila melanogaster)
Reactivity:
Yeast
Reactivity:
Yeast (Saccharomyces cerevisiae)
Reactivity:
Plant
Reactivity:
Barnyard Grass (Echinochloa)
Reactivity:
Beet (Beta vulgaris)
Reactivity:
Cotton (Gossypium)
Reactivity:
Mung Bean (Vigna radiata)
Reactivity:
Grass (Sorghum)
Reactivity:
Wheat (Triticum spp.)
Host Species:
Mouse
Isotype:
IgG2b
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Description

HDEL Antibody [2E7]

HDEL Antibody [2E7] is a mouse monoclonal antibody directed against HDEL, offered by Assay Genie for research applications. Reported applications include WB and ICC/IF. Reported reactivity: Fruit Fly (Drosophila melanogaster), Yeast, Yeast (Saccharomyces cerevisiae), Plant, Barnyard Grass (Echinochloa), Beet (Beta vulgaris), Cotton (Gossypium), Mung Bean (Vigna radiata), Grass (Sorghum) and Wheat (Triticum spp.). Supplied as IgG2b, purified by Protein G.

The HDEL sequence (His-Asp-Glu-Leu) is a C-terminal ER retention signal found in many soluble endoplasmic reticulum (ER) resident proteins. It plays a critical role in maintaining ER homeostasis by ensuring that chaperones and folding enzymes are retained or retrieved from the Golgi apparatus back to the ER. In neurodegenerative diseases, where ER stress and protein misfolding are prominent pathological features, the HDEL motif is essential for the proper localization and function of ER-resident proteins such as GRP78, GRP94, and protein disulfide isomerases. Disruption of HDEL-mediated retention can impair the unfolded protein response (UPR), exacerbate proteotoxic stress, and contribute to neuronal dysfunction. Moreover, the efficiency of the HDEL retrieval system may decline with age or under chronic stress, potentially leading to the mislocalization of protective chaperones and increased vulnerability to neurodegeneration. Understanding the dynamics of HDEL-mediated trafficking offers insights into ER stress regulation and may inform therapeutic strategies aimed at enhancing proteostasis in neurodegenerative disorders. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.

Product Name:HDEL Antibody [2E7]
Product SKU:STMA00046
Size:100 µg
Target:HDEL
Host Species:Mouse
Antibody Type:Monoclonal
Clone ID:2E7
Research Areas:Cancer, Heat Shock, Cell Signaling, Protein Trafficking
Buffer:PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB, ICC/IF
Antibody Isotype:IgG2b
Purification:Protein G Purified
Concentration:1 mg/ml
Reactivity:Fruit Fly (Drosophila melanogaster), Yeast, Yeast (Saccharomyces cerevisiae), Plant, Barnyard Grass (Echinochloa), Beet (Beta vulgaris), Cotton (Gossypium), Mung Bean (Vigna radiata), Grass (Sorghum), Wheat (Triticum spp.)
Recommended Dilution:WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Specificity:Detects ~78kDa.
Guarantee:12 months from date of dispatch
Immunogen:Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
Immunogen Species:Yeast
Cellular Localization:Endoplasmic Reticulum
Alternative Names:H-D-E-L (his-asp-glu-leu), endoplasmic reticulum, luminal ER protein retention, KDELR1, Endoplasmic reticulum retention signal
References:1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697. 3. Entrez Gene: HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.
Note:For research use only.
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