Cofilin Rabbit Monoclonal Antibody (CAB2658)

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SKU:
CAB2658
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
CFL, cofilin, HEL-S-15, Cofilin
Research Use:
Cell Biology
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Description

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Cofilin Monoclonal Antibody (CAB2658)

The Cofilin Monoclonal Antibody (CAB2658) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, derived from rabbit immunization, demonstrates high reactivity with cofilin protein in human samples and has been validated for Western blot applications. By specifically binding to cofilin, this antibody enables precise detection and analysis of cofilin expression in a variety of cell types, making it an essential component for studies in cell biology and cancer research.Cofilin is a critical protein involved in the reorganization of actin filaments, playing a crucial role in cell migration, division, and signaling processes.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Cofilin Monoclonal Antibody
SKU:CAB2658
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC2615
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MASG VAVS DGVI KVFN DMKV RKSS TPEE VKKR KKAV LFCL SEDK KNII LEEG KEIL VGDV GQTV DDPY ATFV KMLP DKDC RYAL YDAT YETK ESKK EDLV
Tested Applications:WB ELISA
Recommended Dilution:
WB1:1000 - 1:4000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CFL, cofilin, HEL-S-15, Cofilin
Positive Sample:293T, MCF7, NIH/3T3, Mouse thymus, Rat brain
Cellular Localization:Cell Projection, Cytoplasm, Cytoplasmic Side, Nucleus Matrix, Peripheral Membrane Protein, Cytoskeleton, Lamellipodium Membrane, Ruffle Membrane.
Calculated MW:19kDa
Observed MW:19kDa

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

Purification MethodAffinity purification
Gene ID1072
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Cofilin Rabbit mAb (CAB2658) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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