CaMKII Rabbit Monoclonal Antibody (CAB0198)

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SKU:
CAB0198
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Reactivity:
Human, Mouse, Rat
Synonyms:
CAM2, CAMK2, CAMKB, MRD54, CaMKIIbeta, CaMKII
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Description

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CaMKII Monoclonal Antibody (CAB0198)

The CaMKII Monoclonal Antibody (CAB0198) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been extensively validated for Western blot applications. By binding specifically to the CAMKII protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential reagent for investigations in neuroscience and cell biology.CAMKII, also known as calcium/calmodulin-dependent protein kinase II, plays a crucial role in synaptic plasticity and memory formation in the brain.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CaMKII Monoclonal Antibody
SKU:CAB0198
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC1814
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:GVIL YILL VGYP PFWD EDQH RLYQ QIKA GAYD FPSP EWDT VTPE AKDL INKM LTIN PSKR ITAA EALK HPWI SHRS TVAS CMHR QETV DCLK KFNA RRKL K
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:1000 - 1:2000
IHC-P1:200 - 1:800
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CAM2, CAMK2, CAMKB, MRD54, CaMKIIbeta, CaMKII
Positive Sample:SH-SY5Y, U-251MG, Mouse brain, Rat brain, C6
Cellular Localization:Cytoplasm, Cytoplasmic Side, Peripheral Membrane Protein, Sarcoplasmic Reticulum Membrane, Centrosome, Cytoskeleton, Microtubule Organizing Center.
Calculated MW:73kDa
Observed MW:50kDa/60kDa

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID816
RRIDAB_2861457
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using CaMKII Rabbit mAb (CAB0198) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageWestern blot analysis of various lysates using CaMKII Rabbit mAb (CAB0198) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (STMA00016). Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (STMA00016) at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT.
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Anti-CaMKII Antibody [22B1]

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CaMKII Antibody [22B1]CaMKII Antibody [22B1] is a mouse monoclonal antibody directed against CaMKII, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF,...