The ACTN3 Monoclonal Antibody (CAB19631) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By binding specifically to the ACTN3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent tool for studying muscle biology, exercise physiology, and sports genetics.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ACTN3 Monoclonal Antibody
SKU:
CAB19631
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2202
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
DRLE GDHQ LLQE SLVF DNKH TVYS MEHI RVGW EQLL TSIA RTIN EVEN QVLT RDAK GLSQ EQLN EFRA SFNH FDRK QNGM MEPD DFRA CLIS MGYD LGEV E
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:1000 - 1:2000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACTN3D, ACTN3
Positive Sample:
Mouse skeletal muscle, Rat skeletal muscle
Cellular Localization:
Cortical Actin Cytoskeleton, Cytosol, Extracellular Exosome, Plasma Membrane, Z Disc.
Calculated MW:
103kDa
Observed MW:
103kDa
This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.
Purification Method
Affinity purification
Gene ID
89
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ACTN3 Rabbit mAb (CAB19631) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of paraffin-embedded Rat skeletal muscle tissue using ACTN3 Rabbit mAb (CAB19631) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
