The Actin (plant specific) Mouse mAb (CABC009) is a high-quality antibody developed for reliable detection and analysis of target proteins. Plant actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins, playing key roles in cell motility, structural integrity, and cell architecture in plant systems. It is highly conserved across eukaryotes and widely used as a reference protein in plant molecular biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Various Plants samples.
Product Name:
Actin (plant specific) Mouse mAb
SKU:
CABC009
Size:
100μL, 200μL
Reactivity:
Various Plants
Clone Number:
AMC0496
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:10000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Purification Method
Affinity purification
RRID
AB_2771701
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 1% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Arabidopsis thaliana tissue using Actin (plant specific) Mouse mAb (CABC009) at 1:2000-1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of various lysates using Actin (plant specific) Mouse mAb (CABC009) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
