The ACTN2 Antibody (CAB3718) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to Alpha-Actinin-2, this antibody enables precise detection and analysis of the protein in various cell types, making it an ideal choice for studies in cell biology and cancer research.Alpha-Actinin-2 plays a crucial role in maintaining cell structure and function, particularly in processes like cell movement and signaling.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ACTN2 Antibody
SKU:
CAB3718
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MPD6, CMH23, CMYP8, CMD1AA, MYOCOZ, ACTN2
Positive Sample:
SH-SY5Y, HT-29, HeLa, Mouse heart, Mouse skeletal muscle, Mouse lung, Rat liver, Rat heart
Cellular Localization:
Cytoplasm, Z Line, Myofibril, Sarcomere.
Calculated MW:
104kDa
Observed MW:
104kDa
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
88
RRID
AB_2765228
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ACTN2 Rabbit pAb (CAB3718) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using ACTN2 Rabbit pAb (CAB3718) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
system_update_altDatasheetHuman Actinin Alpha 2 (ACTN2) ELISA KitThe Human Actinin Alpha 2 (ACTN2) ELISA Kit is specifically designed for the quantitative detection of Actinin
