The ACSS3 Antibody (CAB13781) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through immunization of rabbits, specifically binds to ACSS3 in human samples and is validated for use in Western blot applications. By enabling the detection and analysis of ACSS3 in various cell types, this antibody is essential for studies in metabolism, cancer, and other related fields.ACSS3, also known as acetyl-CoA synthetase 3, is essential for the production of acetyl-CoA, a critical molecule in multiple metabolic pathways. Dysregulation of ACSS3 has been implicated in various diseases, including cancer and metabolic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ACSS3 Antibody
SKU:
CAB13781
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACSS3
Positive Sample:
Mouse kidney, Mouse lung, Rat kidney, Rat liver
Cellular Localization:
Mitochondrion.
Calculated MW:
75kDa
Observed MW:
75kDa
Enables propionate-CoA ligase activity. Predicted to be involved in ketone body biosynthetic process. Located in mitochondrial matrix.
Purification Method
Affinity purification
Gene ID
79611
RRID
AB_2760639
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACSS3 Rabbit pAb (CAB13781) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
