The Acetyl-Histone H3-K4 Antibody (CAB16078) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbits, is highly specific to the acetylated form of Histone H3 at lysine 4 (K4) and is suitable for use in various applications, including Western blotting and immunohistochemistry.Histone acetylation at lysine 4 plays a crucial role in gene regulation and chromatin structure, influencing a wide range of cellular processes such as transcription, DNA repair, and apoptosis. The Acetyl-Histone H3 (K4) Antibody enables the detection and analysis of this specific histone modification, providing valuable insights into epigenetic mechanisms underlying gene expression and cellular function.
This antibody is validated for use in WB, IF/ICC, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Acetyl-Histone H3-K4 Antibody
SKU:
CAB16078
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ARTK Q
Tested Applications:
WBIF/ICCChIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
C6 treated with TSA , NIH/3T3 treated with TSA , HeLa treated with TSA
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
15kDa
Observed MW:
17kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2763519
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells using Acetyl-Histone H3-K4 Rabbit pAb (CAB16078) at 1:400 dilution. NIH/3T3 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from C6 cells using Acetyl-Histone H3-K4 Rabbit pAb (CAB16078) at 1:400 dilution. C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K4 Rabbit pAb (CAB16078) at dilution of 1:100.C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K4 Rabbit pAb (CAB16078) at dilution of 1:100.HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 antibody (CAB16078) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
