[KO Validated] NT5E/CD73 Rabbit Polyclonal Antibody (CAB2029)

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SKU:
CAB2029
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
NT, eN, NT5, NTE, eNT, CD73, E5NT, CALJA, 5E
Research Use:
Cell Biology
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Description

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[KO Validated] NT5E/CD73 Antibody (CAB2029)

The [KO Validated] NT5E/CD73 Antibody (CAB2029) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By targeting the 5'-Nucleotidase protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an indispensable tool for investigations in fields such as biochemistry, cell biology, and drug development.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] NT5E/CD73 Antibody
SKU:CAB2029
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:WELT ILHT NDVH SRLE QTSE DSSK CVNA SRCM GGVA RLFT KVQQ IRRA EPNV LLLD AGDQ YQGT IWFT VYKG AEVA HFMN ALRY DAMA LGNH EFDN GVEG LIEP LLKE AKFP ILSA NIKA KGPL ASQI SGLY LPYK VLPV GDEV VGIV GYTS KETP FLSN PGTN LVFE DEIT ALQP EVDK LKTL NVNK IIAL GHSG FEMD KLIA QKVR GVDV VVGG HSNT FLYT GNPP SKEV PAGK YPFI VTSD DGRK VPVV QAYA FGKY LGYL KIEF DERG NVIS SHGN PILL NSSI PEDP SIKA DINK WRIK LDNY STQE LGKT IVYL DGSS QSCR FREC NMGN LICD AMIN NNLR HTD
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:3000 - 1:30000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NT, eN, NT5, NTE, eNT, CD73, E5NT, CALJA, 5E
Positive Sample:Mouse spleen, A-431, A-375, Rat uterus
Cellular Localization:Cell Membrane, Gpi-Anchor, Lipid-Anchor.
Calculated MW:63kDa
Observed MW:70kDa

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID4907
RRIDAB_2764053
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using CD73/NT5E Rabbit pAb (CAB2029)at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): K-562 Exposure time: 10s.
Product ImageWestern blot analysis of lysates from Mouse spleen using CD73/NT5E Rabbit pAb (CAB2029) at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] CD73/NT5E Rabbit pAb (CAB2029) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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