SUCLG2 Antibody (PACO58364)
- SKU:
- PACO58364
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | SUCLG2 Antibody (PACO58364) |
抗体コード: | PACO58364 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
反応性: | Human |
免疫原: | Recombinant Human Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial protein (161-292AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, Raji whole cell lysate, LO2 whole cell lysate, U87 whole cell lysate. All lanes: SUCLG2 antibody at 6.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 48 kDa. Observed band size: 47 kDa. | |
IHC image of PACO58364 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO58364 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | GTP-specific succinyl-CoA synthetase functions in the citric acid, cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The β subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the α subunit. |
シノニム: | Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial (EC 6.2.1.4) (GTP-specific succinyl-CoA synthetase subunit beta) (G-SCS) (GTPSCS) (Succinyl-CoA synthetase beta-G chain) (SCS-betaG), SUCLG2 |
UniProt Protein Function: | SUCLG2: Catalyzes the GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Belongs to the succinate/malate CoA ligase beta subunit family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - propanoate; EC 6.2.1.4; Ligase; Mitochondrial Chromosomal Location of Human Ortholog: 3p14.1 Cellular Component: mitochondrial matrix; mitochondrion; plasma membrane Biological Process: tricarboxylic acid cycle |
NCBI Summary: | This gene encodes a GTP-specific beta subunit of succinyl-CoA synthetase. Succinyl-CoA synthetase catalyzes the reversible reaction involving the formation of succinyl-CoA and succinate. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 5 and 12. [provided by RefSeq, Apr 2010] |
UniProt Code: | Q96I99 |
NCBI GenInfo Identifier: | 294862256 |
NCBI Gene ID: | 8801 |
NCBI Accession: | NP_001171070.1 |
UniProt Secondary Accession: | Q96I99,O95195, Q6NUH7, Q86VX8, Q8WUQ1, C9JVT2, |
UniProt Related Accession: | Q96I99 |
Molecular Weight: | 56.3kD |
NCBI Full Name: | succinate--CoA ligase |
NCBI Synonym Full Names: | succinate-CoA ligase GDP-forming beta subunit |
NCBI Official Symbol: | SUCLG2 |
NCBI Official Synonym Symbols: | G-SCS; GBETA; GTPSCS |
NCBI Protein Information: | succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial; succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial |
UniProt Protein Name: | Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial |
UniProt Synonym Protein Names: | GTP-specific succinyl-CoA synthetase subunit beta |
UniProt Gene Name: | SUCLG2 |
Antibodies |
Anti-SUCLG2 Antibody (CAB8976) |
Anti-SUCLG2 Antibody (CAB19872)[KO Validated] |
SUCLG2 Antibody (PACO12573) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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