RIPK3 Antibody (PACO42842)
- SKU:
- PACO42842
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | RIPK3 Antibody (PACO42842) |
抗体コード: | PACO42842 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:200-1:500 |
反応性: | Human |
免疫原: | Recombinant Human Receptor-interacting serine/threonine-protein kinase 3 protein (1-518AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO42842 at dilution of 1:100. | |
Immunofluorescence staining of Hela cells with PACO42842 at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Western Blot. Positive WB detected in: HepG2 whole cell lysate. All lanes: RIPK3 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57, 28, 26 kDa. Observed band size: 57 kDa. |
バックグラウンド: | Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-α family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid, cycle and oxidative phosphorylation, which could result in enhanced ROS production. |
シノニム: | Receptor-interacting serine/threonine-protein kinase 3 (EC 2.7.11.1) (RIP-like protein kinase 3) (Receptor-interacting protein 3) (RIP-3), RIPK3, RIP3 |
UniProt Protein Function: | RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; RIPK family Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: mitochondrion; plasma membrane; cytosol Molecular Function:NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; identical protein binding; protein binding; transcription coactivator activity; protein complex binding; ATP binding; protein kinase activity Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; regulation of activated T cell proliferation; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; MyD88-independent toll-like receptor signaling pathway; T cell differentiation in the thymus; protein modification process; toll-like receptor 3 signaling pathway; signal transduction; lymph node development; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; toll-like receptor signaling pathway; positive regulation of interferon type I production; activation of protein kinase activity; innate immune response; T cell homeostasis; positive regulation of ligase activity; toll-like receptor 4 signaling pathway; protein homooligomerization; positive regulation of oxidoreductase activity |
UniProt Code: | Q9Y572 |
NCBI GenInfo Identifier: | 40254844 |
NCBI Gene ID: | 11035 |
NCBI Accession: | NP_006862 |
UniProt Secondary Accession: | Q9Y572,Q5J795, Q5J796, Q6P5Y1, B4DJL9, C4AM87, |
UniProt Related Accession: | Q9Y572 |
Molecular Weight: | 25,326 Da |
NCBI Full Name: | receptor-interacting serine/threonine-protein kinase 3 |
NCBI Synonym Full Names: | receptor-interacting serine-threonine kinase 3 |
NCBI Official Symbol: | RIPK3 |
NCBI Official Synonym Symbols: | RIP3 |
NCBI Protein Information: | receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3; receptor interacting protein 3; receptor-interacting protein 3 |
UniProt Protein Name: | Receptor-interacting serine/threonine-protein kinase 3 |
UniProt Synonym Protein Names: | RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3 |
Protein Family: | Putative zinc metalloprotease |
UniProt Gene Name: | RIPK3 |
UniProt Entry Name: | RIPK3_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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