RALBP1 Antibody (PACO52338)
- SKU:
- PACO52338
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | RALBP1 Antibody (PACO52338) |
抗体コード: | PACO52338 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human RalA-binding protein 1 protein (6-105AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: K562 whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate. All lanes: RALBP1 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 77 kDa. Observed band size: 77 kDa. | |
Immunohistochemistry of paraffin-embedded human tonsil tissue using PACO52338 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human breast cancer using PACO52338 at dilution of 1:100. |
バックグラウンド: | Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin. |
シノニム: | RalA-binding protein 1 (RalBP1) (76 kDa Ral-interacting protein) (Dinitrophenyl S-glutathione ATPase) (DNP-SG ATPase) (Ral-interacting protein 1), RALBP1, RLIP1 RLIP76 |
UniProt Protein Function: | RALBP1: Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin. Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDK1 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis. Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes. |
UniProt Protein Details: | Protein type:GAPs; GAPs, Rac/Rho Chromosomal Location of Human Ortholog: 18p11.3 Cellular Component: membrane; cytosol Molecular Function:protein binding; ATPase activity, coupled to movement of substances; ATPase activity; Rac GTPase binding; Ral GTPase binding; GTPase activator activity Biological Process: regulation of GTPase activity; regulation of small GTPase mediated signal transduction; metabolic process; transport; small GTPase mediated signal transduction; chemotaxis; signal transduction |
NCBI Summary: | RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).[supplied by OMIM, Nov 2010] |
UniProt Code: | Q15311 |
NCBI GenInfo Identifier: | 34098413 |
NCBI Gene ID: | 10928 |
NCBI Accession: | Q15311.3 |
UniProt Related Accession: | Q15311 |
Molecular Weight: | |
NCBI Full Name: | RalA-binding protein 1 |
NCBI Synonym Full Names: | ralA binding protein 1 |
NCBI Official Symbol: | RALBP1 |
NCBI Official Synonym Symbols: | RIP1; RLIP1; RLIP76 |
NCBI Protein Information: | ralA-binding protein 1 |
UniProt Protein Name: | RalA-binding protein 1 |
UniProt Synonym Protein Names: | 76 kDa Ral-interacting protein; Dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; Ral-interacting protein 1 |
Protein Family: | RNA-binding protein |
UniProt Gene Name: | RALBP1 |
UniProt Entry Name: | RBP1_HUMAN |