PPP2CA Antibody (PACO29132)
- SKU:
- PACO29132
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | PPP2CA Antibody (PACO29132) |
抗体コード: | PACO29132 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human Serine/threonine-protein phosphatase 2A catalytic subunit α isoform protein (1-309AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: PPP2CA antibody at 2.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36, 30 kDa Observed band size: 36, 30 kDa . | |
Immunofluorescent analysis of Hela cells using PACO29132 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human liver cancer using PACO29132 at dilution of 1:100. |
バックグラウンド: | PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. |
シノニム: | Serine/threonine-protein phosphatase 2A catalytic subunit α isoform (PP2A-alpha) (EC 3.1.3.16) (Replication protein C) (RP-C), PPP2CA |
UniProt Protein Function: | PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. |
UniProt Protein Details: | Protein type:EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region Molecular Function:protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of cell differentiation; regulation of growth; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein catabolic process; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process |
NCBI Summary: | This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008] |
UniProt Code: | P67775 |
NCBI GenInfo Identifier: | 54038809 |
NCBI Gene ID: | 5515 |
NCBI Accession: | P67775.1 |
UniProt Related Accession: | P67775 |
Molecular Weight: | |
NCBI Full Name: | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
NCBI Synonym Full Names: | protein phosphatase 2 catalytic subunit alpha |
NCBI Official Symbol: | PPP2CA |
NCBI Official Synonym Symbols: | RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha |
NCBI Protein Information: | serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
UniProt Protein Name: | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
UniProt Synonym Protein Names: | Replication protein C |
Protein Family: | PP2A regulatory |
UniProt Gene Name: | PPP2CA |
UniProt Entry Name: | PP2AA_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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