MARK2 Antibody (PACO55190)
- SKU:
- PACO55190
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | MARK2 Antibody (PACO55190) |
抗体コード: | PACO55190 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:2000-1:10000, IHC:1:20-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Serine/threonine-protein kinase MARK2 protein (373-594AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO55190 at dilution of 1:100. | |
Western Blot. Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate. All lanes: MARK2 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 88, 79, 84, 82, 81, 78, 87, 83, 80 kDa. Observed band size: 88 kDa. |
バックグラウンド: | Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4, MAPT/TAU, and RAB11FIP2. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. |
シノニム: | Serine/threonine-protein kinase MARK2 (EC 2.7.11.1) (EC 2.7.11.26) (ELKL motif kinase 1) (EMK-1) (MAP/microtubule affinity-regulating kinase 2) (PAR1 homolog) (PAR1 homolog b) (Par-1b) (Par1b), MARK2, EMK1 |
UniProt Protein Function: | MARK2: a CAMK protein kinase of the CAMKL group. Plays a role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. 12 isoforms of the human protein are produced by alternative promoter. |
UniProt Protein Details: | Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.26; CAMK group; CAMKL family; MARK subfamily Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: membrane; mitochondrion; plasma membrane; actin filament; basal cortex; nucleus; lateral plasma membrane Molecular Function:protein serine/threonine kinase activity; protein binding; tau-protein kinase activity; magnesium ion binding; protein kinase activator activity; lipid binding; ATP binding Biological Process: establishment and/or maintenance of epithelial cell polarity; Wnt receptor signaling pathway; establishment of cell polarity; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; mitochondrion degradation; neuron migration; activation of protein kinase activity; mitochondrion localization; regulation of axonogenesis; protein amino acid phosphorylation; regulation of cytoskeleton organization and biogenesis |
NCBI Summary: | This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009] |
UniProt Code: | Q7KZI7 |
NCBI GenInfo Identifier: | 62510922 |
NCBI Gene ID: | 2011 |
NCBI Accession: | Q7KZI7.2 |
UniProt Secondary Accession: | Q7KZI7,Q15449, Q15524, Q5XGA3, Q68A18, Q96HB3, Q96RG0 |
UniProt Related Accession: | Q7KZI7 |
Molecular Weight: | 788 |
NCBI Full Name: | Serine/threonine-protein kinase MARK2 |
NCBI Synonym Full Names: | MAP/microtubule affinity-regulating kinase 2 |
NCBI Official Symbol: | MARK2 |
NCBI Official Synonym Symbols: | EMK1; EMK-1; PAR-1; Par1b; Par-1b |
NCBI Protein Information: | serine/threonine-protein kinase MARK2; PAR1 homolog b; ELKL motif kinase 1; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK |
UniProt Protein Name: | Serine/threonine-protein kinase MARK2 |
UniProt Synonym Protein Names: | ELKL motif kinase 1; EMK-1; MAP/microtubule affinity-regulating kinase 2; PAR1 homolog; PAR1 homolog b; Par-1b; Par1b |
Protein Family: | Serine/threonine-protein kinase |
UniProt Gene Name: | MARK2 |
UniProt Entry Name: | MARK2_HUMAN |
Antibodies |
Anti-MARK2 Antibody (CAB16706) |
MARK2 Antibody (PACO01043) |
Phospho-MARK2 (T596) Antibody (PACO03615) |
MARK2 Antibody (PACO03616) |
MARK2 Antibody (PACO10394) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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