Description
| Product Name: | Human TPPP Recombinant Protein |
| Product Code: | RPPB4993 |
| Size: | 10µg |
| Species: | Human |
| Target: | TPPP |
| Synonyms: | Tubulin polymerization-promoting protein, TPPP, 25 kDa brain-specific protein, TPPP/p25, p24, p25-alpha, TPPP1, p25, p25alpha. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | TPPP filtered (0.4 �m) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4. |
| Solubility: | It is recommended to add 200�l deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. TPPP is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MKHHHHHHASMADKAKPAKA ANRTPPKSPG DPSKDRAAKR LSLESEGAGE GAAASPELSA LEEAFRRFAV HGDARATGRE MHGKNWSKLC KDCQVIDGRN VTVTDVDIVF SKIKGKSCRT ITFEQFQEAL EELAKKRFKD KSSEEAVREV HRLIEGKAPI ISGVTKAISS PTVSRLTDTT KFTGSHKERF DPSGKGKGKA GRVDLVDESG YVSGYKHAGT YDQKVQGGK |
Tubulin Polymerization Promoting Protein (TPPP) probably plays a part in the polymerization of tubulin into microtubules, as well as in microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. TPPP may also have a role in mitotic spindle assembly and nuclear envelope breakdown. TPPP/p235 level in cerebrospinal fluid is considerably higher in multiple sclerosis patients.
TPPP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 229 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 24.9kDa (calculated).
| UniProt Protein Function: | TPPP: May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown. Belongs to the TPPP family. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 5p15.3 Cellular Component: cytoplasm; microtubule; nucleus; perinuclear region of cytoplasm Molecular Function:microtubule binding; protein binding; tubulin binding Biological Process: microtubule bundle formation; microtubule polymerization; positive regulation of protein complex assembly; positive regulation of protein polymerization |
| UniProt Code: | O94811 |
| NCBI GenInfo Identifier: | 28380097 |
| NCBI Gene ID: | 11076 |
| NCBI Accession: | O94811.1 |
| UniProt Related Accession: | O94811 |
| Molecular Weight: | 23,694 Da |
| NCBI Full Name: | Tubulin polymerization-promoting protein |
| NCBI Synonym Full Names: | tubulin polymerization promoting protein |
| NCBI Official Symbol: | TPPP�� |
| NCBI Official Synonym Symbols: | p24; p25; TPPP1; TPPP/p25; p25alpha�� |
| NCBI Protein Information: | tubulin polymerization-promoting protein |
| UniProt Protein Name: | Tubulin polymerization-promoting protein |
| UniProt Synonym Protein Names: | 25 kDa brain-specific protein; TPPP/p25; p24; p25-alpha |
| Protein Family: | TPPP family protein |
| UniProt Gene Name: | TPPP�� |
| UniProt Entry Name: | TPPP_HUMAN |
