Human TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
The Human TIMP-1 (Tissue Inhibitors of Metalloproteinase-1) ELISA Kit is a powerful tool for the precise measurement of TIMP-1 levels in human biological samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it suitable for various research purposes.TIMP-1 is a key protein that regulates the activity of metalloproteinases, which are enzymes involved in tissue remodeling and cell migration. Dysregulation of TIMP-1 has been linked to various diseases, including cancer, arthritis, and cardiovascular disorders, highlighting its importance as a potential therapeutic target and diagnostic marker.
With the Human TIMP-1 ELISA Kit, researchers can effectively study the role of TIMP-1 in disease pathogenesis and progression, leading to valuable insights for developing novel treatment strategies. Order your kit today from AssayGenie and accelerate your research efforts in the field of metalloproteinase regulation.
Product Name:
Human TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
SKU:
HUES01451
Target:
Human TIMP-1 (Tissue Inhibitors of Metalloproteinase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TIMP-1. You can calculate the concentration of Human TIMP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
99-113
86-98
Average (%)
92
106
92
1:4
Range (%)
96-110
85-98
92-109
Average (%)
102
91
100
1:8
Range (%)
98-113
86-100
91-108
Average (%)
105
92
98
1:16
Range (%)
95-111
83-96
95-112
Average (%)
101
88
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.4
3.6
0.5
1.4
3.5
Standard deviation
0.03
0.07
0.11
0.03
0.06
0.11
C V (%)
6
5.0
3.06
6.0
4.29
3.14
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TIMP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TIMP-1 in samples. No significant cross-reactivity or interference between Human TIMP-1 and analogues was observed.
