CHKA Antibody (PACO54702)
- SKU:
- PACO54702
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | CHKA Antibody (PACO54702) |
抗体コード: | PACO54702 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Choline kinase α protein (79-173AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: MCF-7 whole cell lysate. All lanes: CHKA antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 51 kDa. Observed band size: 53 kDa. | |
Immunofluorescence staining of HepG2 cells with PACO54702 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human lung cancer using PACO54702 at dilution of 1:100. |
バックグラウンド: | Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline. |
シノニム: | Choline kinase α (CK) (EC 2.7.1.32) (CHETK-alpha) (Ethanolamine kinase) (EK) (EC 2.7.1.82), CHKA, CHK CKI |
UniProt Protein Function: | CHKA: Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline. Belongs to the choline/ethanolamine kinase family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Lipid Metabolism - glycerophospholipid; EC 2.7.1.82; Kinase, other; EC 2.7.1.32 Chromosomal Location of Human Ortholog: 11q13.2 Cellular Component: cytosol Molecular Function:choline binding; signal transducer activity; protein homodimerization activity; cholinesterase activity; drug binding; ethanolamine kinase activity; choline kinase activity; ATP binding Biological Process: choline metabolic process; CDP-choline pathway; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphatidylcholine biosynthetic process; lipid metabolic process; signal transduction; lipid transport; phosphorylation |
NCBI Summary: | The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | P35790 |
NCBI GenInfo Identifier: | 226694197 |
NCBI Gene ID: | 1119 |
NCBI Accession: | P35790.3 |
UniProt Secondary Accession: | P35790,Q8NE29, |
UniProt Related Accession: | P35790 |
Molecular Weight: | 457 |
NCBI Full Name: | Choline kinase alpha |
NCBI Synonym Full Names: | choline kinase alpha |
NCBI Official Symbol: | CHKA |
NCBI Official Synonym Symbols: | CK; EK; CHK; CKI |
NCBI Protein Information: | choline kinase alpha; CHETK-alpha; ethanolamine kinase |
UniProt Protein Name: | Choline kinase alpha |
UniProt Synonym Protein Names: | CHETK-alpha; Ethanolamine kinase (EC:2.7.1.82); EK |
UniProt Gene Name: | CHKA |
UniProt Entry Name: | CHKA_HUMAN |
Antibodies | ELISA Kits |
Anti-CHKA Antibody (CAB15040) | Human Choline kinase alpha (CHKA) ELISA Kit |
CHKA Antibody (PACO08407) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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