The CDKN1A Polyclonal Antibody (CAB20471) is a valuable tool for researchers studying CDKN1A, a key protein involved in cell cycle regulation and tumor suppression. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the CDKN1A protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for research in cancer biology and cell signaling pathways.CDKN1A, also known as p21, plays a crucial role in controlling cell proliferation and preventing the formation of tumors by inhibiting the activity of cyclin-dependent kinases.
Dysregulation of CDKN1A is commonly associated with various types of cancer, making it an important target for study in cancer research. Understanding the mechanisms by which CDKN1A functions can provide valuable insights into the development of novel therapeutics for cancer treatment.Overall, the CDKN1A Polyclonal Antibody (CAB20471) offers researchers a reliable tool for investigating the role of CDKN1A in cell cycle regulation and cancer development, ultimately contributing to advancements in the field of oncology.
Product Name:
CDKN1A Rabbit Polyclonal Antibody
SKU:
CAB20471
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-159 of mouse CDKN1A (NP_031695.1).
cytoplasm, cytosol, nuclear body, nucleolus, nucleoplasm, nucleus, perinuclear region of cytoplasm
Calculated MW:
18kDa
Observed MW:
21kDa
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at the G1 pahse. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
12575
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CDKN1A antibody (CAB20471) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
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