The ALOX15 Polyclonal Antibody (CAB6864) is a versatile tool for researchers studying the ALOX15 enzyme, which plays a key role in inflammation and lipid metabolism. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to the ALOX15 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential tool for studies in immunology, lipid biology, and inflammatory diseases.
ALOX15, also known as 15-lipoxygenase, is involved in the synthesis of bioactive lipids called eicosanoids, which are important signaling molecules in inflammation and immune responses. Dysregulation of ALOX15 has been implicated in various diseases, including atherosclerosis, asthma, and cancer. By studying the role of ALOX15 in these pathologies, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets for intervention.
Product Name:
ALOX15 Polyclonal Antibody
SKU:
CAB6864
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALOX15 (NP_001131.3).
This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Multiple pseudogenes of this gene have been identified in the human genome.
Purification Method:
Affinity purification
Gene ID:
246
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ALOX15 using ALOX15 Rabbit pAb (CAB6864) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 10s.
