ALOX15 Antibody (PACO62227)
- SKU:
- PACO62227
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | ALOX15 Antibody (PACO62227) |
抗体コード: | PACO62227 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Arachidonate 15-lipoxygenase protein (1-225AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO62227 diluted at 1:400 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of Hela cells with PACO62227 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acid, generating a spectrum of bioactive lipid mediators. Converts arachidonic acid, into 12-hydroperoxyeicosatetraenoic acid, 12-HPETE and 15-hydroperoxyeicosatetraenoic acid, 15-HPETE. Also converts linoleic acid, to 13-hydroperoxyoctadecadienoic acid, May also act on (12S)-hydroperoxyeicosatetraenoic acid, (12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass. |
シノニム: | Arachidonate 15-lipoxygenase (15-LOX) (15-LOX-1) (EC 1.13.11.33) (12/15-lipoxygenase) (Arachidonate 12-lipoxygenase, leukocyte-type) (12-LOX) (EC 1.13.11.31) (Arachidonate omega-6 lipoxygenase), ALOX15, LOG15 |
UniProt Protein Function: | ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; EC 1.13.11.33; Oxidoreductase; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; EC 1.13.11.31 Chromosomal Location of Human Ortholog: 17p13.3 Cellular Component: cytosol; extrinsic to internal side of plasma membrane; lipid particle; membrane; plasma membrane Molecular Function:arachidonate 12-lipoxygenase activity; arachidonate 15-lipoxygenase activity; iron ion binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding Biological Process: apoptotic cell clearance; arachidonic acid metabolic process; bone mineralization; hepoxilin biosynthetic process; inflammatory response; leukotriene metabolic process; lipoxygenase pathway; negative regulation of adaptive immune response; ossification; phosphatidylethanolamine biosynthetic process; positive regulation of actin filament polymerization; wound healing |
UniProt Code: | P16050 |
NCBI GenInfo Identifier: | 126396 |
NCBI Gene ID: | 246 |
NCBI Accession: | P16050.3 |
UniProt Secondary Accession: | P16050,Q8N6R7, Q99657, A8K2P4, B7ZA11, |
UniProt Related Accession: | P16050 |
Molecular Weight: | 70,108 Da |
NCBI Full Name: | Arachidonate 15-lipoxygenase |
NCBI Synonym Full Names: | arachidonate 15-lipoxygenase |
NCBI Official Symbol: | ALOX15 |
NCBI Official Synonym Symbols: | 12-LOX; 15LOX-1; 15-LOX-1 |
NCBI Protein Information: | arachidonate 15-lipoxygenase |
UniProt Protein Name: | Arachidonate 15-lipoxygenase |
UniProt Synonym Protein Names: | 12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type (EC:1.13.11.31 |
UniProt Gene Name: | ALOX15 |
UniProt Entry Name: | LOX15_HUMAN |
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Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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