Description
| 抗体名: | XRCC4 Antibody (PACO21671) |
| 抗体コード: | PACO21671 |
| サイズ: | 100ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
| 反応性: | Human |
| 免疫原: | Synthesized peptide derived from Internal of human XRCC4. |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from COLO205 cells, using XRCC4 antibody. |
| バックグラウンド: | Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. |
| シノニム: | DNA-repair protein XRCC4; X-ray repair cross-complementing protein 4; |
| UniProt Protein Function: | XRCC4: Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (XRCC6/XRCC5) and PRKDC. Seems to interact directly with PRKDC but not with the Ku p70/86 dimer. Interacts with XLF/Cernunnos. Interacts with APTX and APLF. Widely expressed. Belongs to the XRCC4 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 5q14.2 Cellular Component: nucleoplasm; DNA ligase IV complex; centrosome; condensed chromosome; DNA-dependent protein kinase complex; cytosol; nucleus; cell junction Molecular Function:protein C-terminus binding; protein binding; DNA binding; ligase activity Biological Process: pro-B cell differentiation; positive regulation of neurogenesis; central nervous system development; viral reproduction; immunoglobulin V(D)J recombination; in utero embryonic development; isotype switching; T cell differentiation in the thymus; DNA repair; double-strand break repair via nonhomologous end joining; positive regulation of fibroblast proliferation; double-strand break repair; response to gamma radiation; DNA ligation during DNA repair; negative regulation of neuron apoptosis; positive regulation of ligase activity; response to X-ray Disease: Short Stature, Microcephaly, And Endocrine Dysfunction |
| NCBI Summary: | The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq, Sep 2008] |
| UniProt Code: | Q13426 |
| NCBI GenInfo Identifier: | 44888352 |
| NCBI Gene ID: | 7518 |
| NCBI Accession: | Q13426.2 |
| UniProt Secondary Accession: | Q13426,Q9BS72, Q9UP94, A8K3X4, |
| UniProt Related Accession: | Q13426 |
| Molecular Weight: | 336 |
| NCBI Full Name: | DNA repair protein XRCC4 |
| NCBI Synonym Full Names: | X-ray repair complementing defective repair in Chinese hamster cells 4 |
| NCBI Official Symbol: | XRCC4 |
| NCBI Protein Information: | DNA repair protein XRCC4; X-ray repair cross-complementing protein 4; X-ray repair, complementing defective, repair in Chinese hamster |
| UniProt Protein Name: | DNA repair protein XRCC4 |
| UniProt Synonym Protein Names: | X-ray repair cross-complementing protein 4 |
| Protein Family: | Xrcc4-like factor |
| UniProt Gene Name: | XRCC4 |
| UniProt Entry Name: | XRCC4_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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