The XCP1 Polyclonal Antibody is specifically designed for research involving XCP1, a protein involved in cell death processes and autophagy regulation. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding to the XCP1 protein, this antibody allows for easy detection and analysis in a variety of cell types, making it an ideal tool for studies in cell biology and cancer research.XCP1 plays a crucial role in regulating cell death pathways and maintaining cellular homeostasis. Understanding the function of XCP1 is important in the context of cancer research, as dysregulation of cell death processes can contribute to tumor growth and metastasis.
By studying the role of XCP1, researchers can gain valuable insights into potential therapeutic targets for cancer treatment and other diseases involving abnormal cell death mechanisms.Overall, the XCP1 Polyclonal Antibody is a valuable tool for researchers looking to investigate the role of XCP1 in cell biology and cancer. Its high reactivity and specificity make it a reliable choice for Western blot analyses, providing researchers with accurate and reliable results for their studies.
Product Name:
XCP1 Polyclonal Antibody
SKU:
CAB22853
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Maize
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 157-377 of maize XCP1(NP_001149806.1).
XCP1 is a xylem-specific papain-like cysteine peptidase in Arabidopsis. Results from confocal microscopy and biochemical subcellular fractionation indicated that XCP1 was localized in the vacuole. Ectopic expression of XCP1 resulted in a reduction in plant size in some lines and early leaf senescence, as indicated by early loss of leaf chlorophyll. Reduced plant size was correlated with higher levels of XCP1, as shown by immunoblot and peptidase activity gel analyses. The XCP1 prodomain exhibits exceptionally high similarity (greater than 80%) to the prodomains of papain and other papain-like enzymes isolated from papaya (Carica papaya) laticifers when compared with all other reported papain-like enzymes. The XCP1 and papain to perform common functions as catalysts of autolytic processing following cell death due to programmed suicide or to wounding is discussed.
Purification Method:
Affinity purification
Gene ID:
100283433
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from B73-329 using XCP1 Rabbit pAb(CAB22853) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 45s.
