The WDR73 Polyclonal Antibody (PAC063427) is a valuable tool for researchers studying the WDR73 protein, which is involved in cellular processes such as DNA repair and cell cycle regulation. This antibody, produced in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications.WDR73, also known as WD repeat domain 73, plays a crucial role in maintaining genomic stability and proper cell division. Dysregulation of WDR73 has been linked to various diseases, including cancer and developmental disorders.
By using this antibody to detect and analyze WDR73 protein levels in various cell types, researchers can gain insights into its function and potential implications in disease pathology.Overall, the WDR73 Polyclonal Antibody is a valuable tool for researchers interested in exploring the role of WDR73 in cellular processes and disease states, making it a valuable asset in the fields of molecular biology and cancer research.
Western Blot. Positive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, THP-1 whole cell lysate. All lanes: WDR73 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42 kDa. Observed band size: 42 kDa.
Immunofluorescence staining of SH-SY5Y cells with PACO63427 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO63427 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
May play a role in the regulation of microtubule organization and dynamics.
Synonyms:
WD repeat-containing protein 73, WDR73
UniProt Protein Function:
WDR73: Belongs to the WD repeat WDR73 family.Chromosomal Location of Human Ortholog: 15q25.2Disease: Galloway-mowat Syndrome
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is thought to contain multiple WD40 repeats. WD40 repeats are motifs that contain 40-60 amino acids, and usually end with Trp-Asp (WD). This protein is found in the cytoplasm during interphase, but accumulates at the spindle poles and astral microtubules during mitosis. Reduced expression of this gene results in abnormalities in the size and morphology of the nucleus. Mutations in this gene have been associated with Galloway-Mowat syndrome PMID: 25466283), which is a rare autosomal recessive disorder that affects both the central nervous system and kidneys. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
