Anti-VRK1 抗体 (PACO44854)

抗体名:	VRK1 Antibody (PACO44854)
抗体コード:	PACO44854
サイズ:	50ul
宿主種:	Rabbit
申し込み:	ELISA, WB, IHC
推奨される希釈:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200
反応性:	Human
免疫原:	Recombinant Human Serine/threonine-protein kinase VRK1 protein (257-396AA)

憲法:	Liquid
ストレージバッファ:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
精製方法:	Antigen Affinity Purified
抗体のクローン性:	Polyclonal
アイソタイプ:	IgG
Conjugate:	Non-conjugated

	Western blot. All lanes: DCXR antibody at 4.74µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa..
	Immunohistochemistry of paraffin-embedded human testis tissue using PACO44854 at dilution of 1:100.
	Immunohistochemistry of paraffin-embedded human lung cancer using PACO44854 at dilution of 1:100.

バックグラウンド:

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.

シノニム:

Serine/threonine-protein kinase VRK1 (EC 2.7.11.1) (Vaccinia-related kinase 1), VRK1

UniProt Protein Function:	VRK1: Serine/threonine kinase that phosphorylates Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1); also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA). Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
UniProt Protein Details:	Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, CK1; Protein kinase, Ser/Thr (non-receptor); CK1 group; VRK family Chromosomal Location of Human Ortholog: 14q32 Cellular Component: cytoplasm; cytosol; Golgi stack; nucleolus; nucleoplasm; nucleus Molecular Function:histone serine kinase activity (H3-S10 specific); nucleosomal histone binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity Biological Process: mitotic nuclear envelope disassembly; mitotic nuclear envelope reassembly; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell shape Disease: Pontocerebellar Hypoplasia, Type 1a
NCBI Summary:	This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99986
NCBI GenInfo Identifier:	45593726
NCBI Gene ID:	7443
NCBI Accession:	Q99986.1
UniProt Secondary Accession:	Q99986,Q3SYL2,
UniProt Related Accession:	Q99986
Molecular Weight:	45,476 Da
NCBI Full Name:	Serine/threonine-protein kinase VRK1
NCBI Synonym Full Names:	vaccinia related kinase 1
NCBI Official Symbol:	VRK1
NCBI Official Synonym Symbols:	PCH1; PCH1A
NCBI Protein Information:	serine/threonine-protein kinase VRK1
UniProt Protein Name:	Serine/threonine-protein kinase VRK1
UniProt Synonym Protein Names:	Vaccinia-related kinase 1
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	VRK1
UniProt Entry Name:	VRK1_HUMAN

Antibodies

Anti-VRK1 Antibody (CAB7745)

Anti-VRK1 Antibody (CAB19938)[KO Validated]

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)