Description
| 抗体名: | VPS35 Antibody (PACO44422) |
| 抗体コード: | PACO44422 |
| サイズ: | 50ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Vacuolar protein sorting-associated protein 35 protein (1-250AA) |
| 憲法: | Liquid |
| ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| 精製方法: | Antigen Affinity Purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemistry of paraffin-embedded human ovarian cancer using PACO44422 at dilution of 1:100. |
 | Immunohistochemistry of paraffin-embedded human testis tissue using PACO44422 at dilution of 1:100. |
| バックグラウンド: | Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for endosomal localization of FAM21C. Mediates the association of the CSC with the WASH complex via FAM21. Required for the endosomal localization of TBC1D5. |
| シノニム: | Vacuolar protein sorting-associated protein 35 (hVPS35) (Maternal-embryonic 3) (Vesicle protein sorting 35), VPS35, MEM3 |
| UniProt Protein Function: | VPS35: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Defects in VPS35 are the cause of Parkinson disease type 17 (PARK17). PARK17 is an autosomal dominant, adult- onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. Belongs to the VPS35 family. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 16q12 Cellular Component: retromer complex; lysosomal membrane; early endosome; late endosome; cytosol; endosome Molecular Function:protein binding; protein transporter activity Biological Process: intracellular protein transport; transcytosis; retrograde transport, endosome to Golgi Disease: Parkinson Disease 17 |
| NCBI Summary: | This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96QK1 |
| NCBI GenInfo Identifier: | 25453321 |
| NCBI Gene ID: | 55737 |
| NCBI Accession: | Q96QK1.2 |
| UniProt Secondary Accession: | Q96QK1,Q561W2, Q9H016, Q9H096, Q9H4P3, Q9H8J0, Q9NRS7 Q9NVG2, Q9NX80, Q9NZK2, |
| UniProt Related Accession: | Q96QK1 |
| Molecular Weight: | 92kDa |
| NCBI Full Name: | Vacuolar protein sorting-associated protein 35 |
| NCBI Synonym Full Names: | vacuolar protein sorting 35 homolog (S. cerevisiae) |
| NCBI Official Symbol: | VPS35 |
| NCBI Official Synonym Symbols: | MEM3; PARK17 |
| NCBI Protein Information: | vacuolar protein sorting-associated protein 35; hVPS35; maternal-embryonic 3 |
| UniProt Protein Name: | Vacuolar protein sorting-associated protein 35 |
| UniProt Synonym Protein Names: | Maternal-embryonic 3; Vesicle protein sorting 35 |
| Protein Family: | Vacuolar protein sorting-associated protein |
| UniProt Gene Name: | VPS35 |
| UniProt Entry Name: | VPS35_HUMAN |
