Description
| 抗体名: | VIPAS39 Antibody (PACO42190) |
| 抗体コード: | PACO42190 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IF:1:50-1:200 |
| 反応性: | Human, Mouse |
| 免疫原: | Recombinant Human Spermatogenesis-defective protein 39 homolog protein (1-493AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: VIPAS39 antibody at 12µg/ml. Lane 1: HepG2 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: 293T whole cell lysate. Lane 4: Mouse kidney tissue. Lane 5: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 58, 52 kDa. Observed band size: 58 kDa. |
 | Immunofluorescence staining of Hela cells with PACO42190 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| バックグラウンド: | Proposed to be involved in endosomal maturation implicating in part VPS33B. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical RAB11A-dependent recycling pathway and in the maintenance of the apical-basolateral polarity. May play a role in lysosomal trafficking, probably via association with the core HOPS complex in a discrete population of endosomes; the functions seems to be indepenedent of VPS33B. May play a role in vesicular trafficking during spermatogenesis (By similarity). May be involved in direct or indirect transcriptional regulation of E-cadherin (By similarity). |
| シノニム: | Spermatogenesis-defective protein 39 homolog (hSPE-39) (VPS33B-interacting protein in apical-basolateral polarity regulator) (VPS33B-interacting protein in polarity and apical restriction), VIPAS39, C14orf133 SPE39 VIPAR |
| UniProt Protein Function: | VIPAS39: a protein of unknown function. Two splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 14q24.3 Cellular Component: recycling endosome; cytoplasmic membrane-bound vesicle; cytoplasm; early endosome; late endosome Molecular Function:protein binding Biological Process: intracellular protein transport; transcription, DNA-dependent; regulation of transcription, DNA-dependent; endosome to lysosome transport; spermatogenesis; cell differentiation Disease: Arthrogryposis, Renal Dysfunction, And Cholestasis 2 |
| NCBI Summary: | This gene encodes a protein involved in the sorting of lysosomal proteins. Mutations in this gene are associated with ARCS2 (arthrogryposis, renal dysfunction, and cholestasis-2). Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010] |
| UniProt Code: | Q9H9C1 |
| NCBI GenInfo Identifier: | 41016926 |
| NCBI Gene ID: | 63894 |
| NCBI Accession: | Q9H9C1.1 |
| UniProt Secondary Accession: | Q9H9C1,O95434, Q9H7E1, Q9H9I9, B4DPI6, |
| UniProt Related Accession: | Q9H9C1 |
| Molecular Weight: | 493 |
| NCBI Full Name: | Spermatogenesis-defective protein 39 homolog |
| NCBI Synonym Full Names: | VPS33B interacting protein, apical-basolateral polarity regulator, spe-39 homolog |
| NCBI Official Symbol: | VIPAS39 |
| NCBI Official Synonym Symbols: | SPE39; VIPAR; SPE-39; VPS16B; hSPE-39; C14orf133 |
| NCBI Protein Information: | spermatogenesis-defective protein 39 homolog; protein spe-39 homolog; VPS33B-interacting protein in polarity and apical restriction; VPS33B-interacting protein in apical-basolateral polarity regulator; VPS33B-interacting protein involved in polarity and apical protein restriction |
| UniProt Protein Name: | Spermatogenesis-defective protein 39 homolog |
| UniProt Synonym Protein Names: | VPS33B-interacting protein in apical-basolateral polarity regulator; VPS33B-interacting protein in polarity and apical restriction |
| Protein Family: | Spermatogenesis-defective protein |
| UniProt Gene Name: | VIPAS39 |
| UniProt Entry Name: | SPE39_HUMAN |
