Description
| 抗体名: | USP13 Antibody (PACO23492) |
| 抗体コード: | PACO23492 |
| サイズ: | 100ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100 |
| 反応性: | Human |
| 免疫原: | Synthesized peptide derived from internal of human USP13. |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from mouse brain cells, using USP13 antibody. |
 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using USP13 antibody. |
| バックグラウンド: | Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. |
| シノニム: | Ubiquitin carboxyl-terminal hydrolase 13; EC 3.1.2.15; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13; Deubiquitinating enzyme 13 |
| UniProt Protein Function: | USP13: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease Chromosomal Location of Human Ortholog: 3q26.33 Cellular Component: cytosol; nucleoplasm Molecular Function:chaperone binding; cysteine-type endopeptidase activity; protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity Biological Process: cell proliferation; melanocyte differentiation; protein deubiquitination; protein stabilization; regulation of autophagy; regulation of transcription, DNA-dependent |
| UniProt Code: | Q92995 |
| NCBI GenInfo Identifier: | 209572692 |
| NCBI Gene ID: | 8975 |
| NCBI Accession: | Q92995.2 |
| UniProt Secondary Accession: | Q92995,Q96B25, A8K2S3, B4DYF3, D3DNS2, |
| UniProt Related Accession: | Q92995 |
| Molecular Weight: | 97kDa |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 13 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 13 (isopeptidase T-3) |
| NCBI Official Symbol: | USP13 |
| NCBI Official Synonym Symbols: | ISOT3; IsoT-3 |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 13 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 13 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme 13; Isopeptidase T-3; ISOT-3; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13 |
| UniProt Gene Name: | USP13 |
