Description
system_update_altDatasheet
| Product Name: | TUNEL 1-Step In Situ Apoptosis Kit (FL647) |
| Product Code: | AKES074 |
| Size: | 20T, 50T, 100T |
| Application: | DNA Fragmentation |
| Detection Method: | Fluorometric Method |
| Sample Type: | Paraffin section, frozen section, cell slide |
| Assay Time: | 3 hours |
| Detection Instrument: | Fluorescence Microscope |
| Dye Type: | FL647 |
| Ex/EM (nm): | 650/665 |
| Filter Set: | LED |
| Other Reagents Required: | 4% Paraformaldehyde, 0.2% Triton X-100, PBS, ddH2O, Mounting solution with anti-fluorescence quencher |
When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.
| Storage: | RT/-20°C. Refer to the Manual. |
| Shipping: | Ice pack |
| Expiration: | 12 months |
 | Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained. |
 | Paraffin embedded rat brain was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (red). The cells were counterstained with DAPI (blue). This photo was taken by confocal microscope. |
 | Paraffin embedded rat heart was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (red). The cells were counterstained with DAPI (blue). This photo was taken by confocal microscope. |
 | Paraffin embedded mouse lung was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (red). The cells were counterstained with DAPI (blue). This photo was taken by confocal microscope. |
 | Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells. |
