Description
| Product Name: | TriMethyl-Histone H3-K9 Monoclonal Antibody |
| Product Code: | CAB22295 |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Applications: | Western blotting, Immunofluorescence |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Clone No: | ARC54898 |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Tested Applications: | WB, IF/ICC, IP, ChIP, ChIP-seq, ELISA, DB, CUT&Tag |
| Key Applications: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:2000-1:20000, IF/ICC 1:50-1:200, ChIP 1:50-1:200, ChIP-seq 1:20-1:100, DB 1:2000-1:20000, CUT&Tag 10, ⁵ cells /1 μg |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Positive Samples: | HeLa, C2C12 , C6 |
| Cellular Location: | Chromosome, Nucleus |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
| Immunogen: | A synthetic trimethylated peptide around K9 of human Histone H3 (NP_003520.1). |
| Sequence: | MARTK QTARK STGGK APRKQ LATKA ARKSA PATGG VKKPH RYRPG TVALR EIRRY QKSTE LLIRK LPFQR LVREI AQDFK TDLRF QSSAV MALQE ACEAY |
| Synonyms: | H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A |
| Calculated MW: | 15kDa |
| Observed MW: | 17kDa |
 | Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and TriMethyl-Histone H3-K9 mAb (CAB22295). The ChIP sequencing results indicate the enrichment pattern of TriMethyl-Histone H3-K9 in selected genomic region and representative gene loci (ZNF404,GAPDH), as shown in figure. |
 | CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1 μg TriMethyl-Histone H3-K9 Rabbit mAb (CAB22295), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of TriMethyl-Histone H3-K9 in representative gene loci (MYT1), as shown in figure. |
 | Western blot analysis of various lysates, using TriMethyl-Histone H3-K9 Rabbit mAb (CAB22295) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
 | Dot-blot analysis of all sorts of peptides using TriMethyl-Histone H3-K9 antibody (CAB22295) at 1:20000 dilution. |
 | Confocal imaging of HeLa cells using TriMethyl-Histone H3-K9 Rabbit mAb (CAB22295,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (CABC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x. |
 | Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody (CAB22295) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
