The TMEM214 Polyclonal Antibody (PAC062719) is a valuable tool for researchers studying TMEM214, a protein involved in various cellular processes. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to the TMEM214 protein, this antibody facilitates the detection and analysis of TMEM214 in different cell types, making it an ideal choice for investigations in cell biology and cancer research.
TMEM214, also known as transmembrane protein 214, is implicated in various cellular functions, including membrane trafficking and signal transduction. Its role in these processes suggests its involvement in disease mechanisms and highlights its potential as a therapeutic target for conditions such as cancer and neurological disorders. Studying the function of TMEM214 is crucial for gaining insights into these disease contexts and developing targeted interventions aimed at modulating its activity.
Western Blot. Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate. All lanes: TMEM214 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 78, 73 kDa. Observed band size: 78 kDa.
Immunoprecipitating TMEM214 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of PACO62719 in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000). Lane 2: PACO62719 (5µg) + HepG2 whole cell lysate (0.5mg). Lane 3: HepG2 whole cell lysate (20µg).
Background:
Critical mediator, in cooperation with CASP4, of endoplasmic reticulum-stress induced apoptosis. Required or the activation of CASP4 following endoplasmic reticulum stress.
Synonyms:
Transmembrane protein 214, TMEM214
UniProt Protein Function:
TMEM214: Belongs to the TMEM214 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; Membrane protein, multi-passChromosomal Location of Human Ortholog: 2p23.3Cellular Component: cytoplasm; cytoplasmic microtubule; endoplasmic reticulum; Golgi apparatus
