Description
| 抗体名: | TMEM2 Antibody (PACO60893) |
| 抗体コード: | PACO60893 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Cell surface hyaluronidase protein (734-866AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60893 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of A549 cells with PACO60893 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| バックグラウンド: | Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan of approximately 5 kDa fragments. Acts as a regulator of angiogenesis and heart morphogenesis by mediating degradation of extracellular hyaluronan, thereby regulating VEGF signaling (By similarity). Is very specific to hyaluronan; not able to cleave chondroitin sulfate or dermatan sulfate. |
| シノニム: | Cell surface hyaluronidase (EC 3.2.1.35) (Transmembrane protein 2), TMEM2, KIAA1412 |
| UniProt Protein Function: | TMEM2: May be required for the heart morphogenesis. Belongs to the TMEM2 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 9q21.13 Cellular Component: integral to membrane Biological Process: multicellular organismal development |
| UniProt Code: | Q9UHN6 |
| NCBI GenInfo Identifier: | 209447096 |
| NCBI Gene ID: | 23670 |
| NCBI Accession: | NP_001129292.1 |
| UniProt Secondary Accession: | Q9UHN6,Q5T838, Q5T839, Q5T840, Q5T841, Q8NBP6, Q9P2D5 A6H8W9, B2RTQ6, |
| UniProt Related Accession: | Q9UHN6 |
| Molecular Weight: | 154,374 Da |
| NCBI Full Name: | transmembrane protein 2 isoform b |
| NCBI Synonym Full Names: | transmembrane protein 2 |
| NCBI Official Symbol: | TMEM2 |
| NCBI Protein Information: | transmembrane protein 2 |
| UniProt Protein Name: | Transmembrane protein 2 |
| Protein Family: | Transmembrane protein |
| UniProt Gene Name: | TMEM2 |
| UniProt Entry Name: | TMEM2_HUMAN |
| Antibodies |
| Anti-TMEM2 Antibody (CAB18439) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
