Description
| 抗体名: | TES Antibody (PACO60428) |
| 抗体コード: | PACO60428 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Testin protein (130-260AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate. All lanes: TES antibody at 2.92µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48, 47 kDa. Observed band size: 48 kDa. |
 | Immunofluorescence staining of Hela cells with PACO60428 at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO60428 diluted at 1:800 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth. |
| シノニム: | Testin (TESS), TES |
| UniProt Protein Function: | TES: Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth. Belongs to the prickle / espinas / testin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 7q31.2 Cellular Component: protein complex; focal adhesion; cytoplasm; plasma membrane; nucleus; cell junction Molecular Function:zinc ion binding Biological Process: negative regulation of cell proliferation |
| UniProt Code: | Q9UGI8 |
| NCBI GenInfo Identifier: | 17380320 |
| NCBI Gene ID: | |
| NCBI Accession: | Q9UGI8.1 |
| Molecular Weight: | 46kDa; 47kDa |
| NCBI Full Name: | Testin |
| UniProt Protein Name: | Testin |
| UniProt Synonym Protein Names: | TESS |
| Protein Family: | Tesmin |
| UniProt Gene Name: | TES |
| UniProt Entry Name: | TES_HUMAN |
| Antibodies | ELISA Kits |
| Anti-TES Antibody (CAB7094) | Human Testin ELISA Kit |
| TES Antibody (PACO12698) | Human Testin (TES) ELISA Kit |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
