Description
| 抗体名: | STK24 Antibody (PACO25192) |
| 抗体コード: | PACO25192 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Serine/threonine-protein kinase 24 protein (1-431AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate, HepG2 whole cell lysate All lanes: STK24 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 48 kDa Observed band size: 50 kDa . |
 | Immunofluorescence staining of Hela cells with PACO25192 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human brain tissue using PACO25192 at dilution of 1:100. |
| バックグラウンド: | Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve. |
| シノニム: | Serine/threonine-protein kinase 24 (EC 2.7.11.1) (Mammalian STE20-like protein kinase 3) (MST-3) (STE20-like kinase MST3) [Cleaved into: Serine/threonine-protein kinase 24 36 kDa subunit (Mammalian STE20-like protein kinase 3 N-terminal) (MST3/N); Serine/threonine-protein kinase 24 12 kDa subunit (Mammalian STE20-like protein kinase 3 C-terminal) (MST3/C)], STK24, MST3 STK3 |
| UniProt Protein Function: | MST3: a protein kinase of the STE20 family. Activated by caspase-mediated cleavage |
| UniProt Protein Details: | Protein type:Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; STE group; STE20 family; YSK subfamily Chromosomal Location of Human Ortholog: 13q31.2-q32.3 Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity Biological Process: regulation of axon regeneration; response to hydrogen peroxide; apoptosis; protein amino acid autophosphorylation; signal transduction; induction of apoptosis by oxidative stress; protein amino acid phosphorylation; negative regulation of cell migration; cell structure disassembly during apoptosis |
| NCBI Summary: | This gene encodes a serine/threonine protein kinase that functions upstream of mitogen-activated protein kinase (MAPK) signaling. The encoded protein is cleaved into two chains by caspases; the N-terminal fragment (MST3/N) translocates to the nucleus and promotes programmed cells death. There is a pseudogene for this gene on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013] |
| UniProt Code: | Q9Y6E0 |
| NCBI GenInfo Identifier: | 13626607 |
| NCBI Gene ID: | 8428 |
| NCBI Accession: | Q9Y6E0.1 |
| UniProt Secondary Accession: | Q9Y6E0,O14840, Q5JV92, |
| UniProt Related Accession: | Q9Y6E0 |
| Molecular Weight: | 47,913 Da |
| NCBI Full Name: | Serine/threonine-protein kinase 24 |
| NCBI Synonym Full Names: | serine/threonine kinase 24 |
| NCBI Official Symbol: | STK24 |
| NCBI Official Synonym Symbols: | MST3; STK3; MST3B; STE20; HEL-S-95 |
| NCBI Protein Information: | serine/threonine-protein kinase 24; STE20-like kinase 3; STE20-like kinase MST3; sterile 20-like kinase 3; epididymis secretory protein Li 95; mammalian STE20-like protein kinase 3; serine/threonine kinase 24 (STE20 homolog, yeast) |
| UniProt Protein Name: | Serine/threonine-protein kinase 24 |
| UniProt Synonym Protein Names: | Mammalian STE20-like protein kinase 3; MST-3; STE20-like kinase MST3Cleaved into the following 2 chains:Serine/threonine-protein kinase 24 36 kDa subunitAlternative name(s):Mammalian STE20-like protein kinase 3 N-terminal; MST3/N |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | STK24 |
| UniProt Entry Name: | STK24_HUMAN |
