The SSH3 Monoclonal Antibody (PAC057340) is a key tool for researchers studying the SSH3 protein, a crucial regulator of cell signaling pathways involved in cancer progression. This specific antibody, produced through monoclonal technology, is highly specific and sensitive to SSH3 protein in human samples. Validated for use in various experimental applications such as Western blot and immunofluorescence, this antibody allows for precise detection and analysis of SSH3 protein levels.SSH3, also known as Src homology 3 domain-containing protein, is known to play a critical role in cell growth, migration, and invasion processes, making it a potential target for cancer therapy research.
Understanding the function and regulation of SSH3 is essential for unraveling its involvement in tumorigenesis and metastasis, offering insights into potential therapeutic interventions. Researchers in the fields of oncology and cell biology will find this antibody invaluable for investigating the role of SSH3 in cancer pathogenesis and identifying novel treatment strategies.
Western Blot. Positive WB detected in: Rat spleen tissue. All lanes: SSH3 antibody at 5.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 73, 53, 57, 43, 17 kDa. Observed band size: 73 kDa.
Immunofluorescence staining of Hela cells with PACO57340 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO57340 diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system.
Background:
Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly (By similarity).
Synonyms:
Protein phosphatase Slingshot homolog 3 (EC 3.1.3.16) (EC 3.1.3.48) (SSH-like protein 3) (SSH-3L) (hSSH-3L), SSH3, SSH3L
UniProt Protein Function:
SSH3: a protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Does not bind to, or colocalize with, filamentous actin. Five alternatively spliced isoforms have been described.Protein type: Cytoskeletal; EC 3.1.3.16; Protein phosphatase, dual-specificity; Phosphatase; Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, Ser/Thr (non-receptor)Chromosomal Location of Human Ortholog: 11q13.2Cellular Component: cytoplasmMolecular Function: actin bindingBiological Process: regulation of actin polymerization and/or depolymerization; regulation of axonogenesis
UniProt Protein Details:
NCBI Summary:
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM, Mar 2008]
