Description
| 抗体名: | SREBF1 Antibody (PACO55794) |
| 抗体コード: | PACO55794 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Sterol regulatory element-binding protein 1 protein (38-201AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO55794 diluted at 1:300 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Western Blot. Positive WB detected in: Jurkat whole cell lysate. All lanes: SREBF1 antibody at 5.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 122, 114, 112, 125, 49, 46 kDa. Observed band size: 122 kDa. |
 | IHC image of PACO55794 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid, and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). |
| シノニム: | Sterol regulatory element-binding protein 1 (SREBP-1) (Class D basic helix-loop-helix protein 1) (bHLHd1) (Sterol regulatory element-binding transcription factor 1) [Cleaved into: Processed sterol regulatory element-binding protein 1], SREBF1, BHLHD1 SREBP1 |
| UniProt Protein Function: | Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). |
| NCBI Summary: | This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Mar 2016] |
| UniProt Code: | P36956 |
| NCBI GenInfo Identifier: | 166897633 |
| NCBI Gene ID: | 6720 |
| NCBI Accession: | P36956.2 |
| UniProt Secondary Accession: | P36956,Q16062, Q59F52, Q6P4R7, Q6PFW7, Q6PJ36, Q8TAK9 B0I4X3, B0I4X4, D3DXC4, |
| UniProt Related Accession: | P36956 |
| Molecular Weight: | 45,816 Da |
| NCBI Full Name: | Sterol regulatory element-binding protein 1 |
| NCBI Synonym Full Names: | sterol regulatory element binding transcription factor 1 |
| NCBI Official Symbol: | SREBF1 |
| NCBI Official Synonym Symbols: | SREBP1; bHLHd1; SREBP1a; SREBP-1c |
| NCBI Protein Information: | sterol regulatory element-binding protein 1 |
| UniProt Protein Name: | Sterol regulatory element-binding protein 1 |
| UniProt Synonym Protein Names: | Class D basic helix-loop-helix protein 1; bHLHd1 |
| UniProt Gene Name: | SREBF1 |
| UniProt Entry Name: | SRBP1_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
