Description
| 抗体名: | SMARCA4 Antibody, Biotin conjugated (PACO46221) |
| 抗体コード: | PACO46221 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA |
| 推奨される希釈: | |
| 反応性: | Human |
| 免疫原: | Recombinant Human Transcription activator BRG1 protein (808-1092AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Biotin |
| バックグラウンド: | Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. |
| シノニム: | Transcription activator BRG1 (EC 3.6.4) (ATP-dependent helicase SMARCA4) (BRG1-associated factor 190A) (BAF190A) (Mitotic growth and transcription activator) (Protein BRG-1) (Protein brahma homolog 1) (SNF2-beta) (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4), SMARCA4, BAF190A BRG1 SNF2B SNF2L4 |
| UniProt Protein Function: | SMARCA4: a member of the SWI/SNF nucleosome remodeling complex with ATP-dependent DNA helicase activity. SWI/SNF complexes are required for mammalian development and are mutated in ~20% of all human primary tumors. Mutated in 35% (13/37) of NSCLC and 5% (1/19) of SCLC human lung cancer cell lines. A component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. Belongs to the neural progenitor-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). Component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Transcriptional coactivator of nuclear hormone receptors. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription, and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. SMARCA4, in consort with ZEB1, represses the transcription of E-cadherin and promotes the epithelial-to-mesenchymal transisition (EMT). SMARCA4 is required for stem cell maintenance in the mouse intestinal epithelium. Its loss inhibits aberrant Wnt-signalling and prevents tumorigenesis in the mouse small intestine. SMARCA4 mutations are the cause of a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood. Belongs to the SNF2/RAD54 helicase family. The human protein includes 5 isoforms produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Tumor suppressor; Transcription, coactivator/corepressor; EC 3.6.1.-; Helicase; EC 3.6.4.-; Nuclear receptor co-regulator; Cell cycle regulation Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: nucleoplasm; heterochromatin; SWI/SNF complex; extracellular space; protein complex; membrane; perichromatin fibrils; nuclear chromatin; nucleolus; nucleus Molecular Function:DNA-dependent ATPase activity; protein binding; nucleosomal DNA binding; androgen receptor binding; Tat protein binding; p53 binding; transcription coactivator activity; protein N-terminus binding; transcription corepressor activity; helicase activity; ATP binding Biological Process: extracellular matrix organization and biogenesis; establishment and/or maintenance of chromatin architecture; glial cell fate determination; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter, mitotic; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; embryonic organ morphogenesis; epidermis morphogenesis; blastocyst hatching; forebrain development; blastocyst growth; vasculogenesis; positive regulation of Wnt receptor signaling pathway; positive regulation of DNA binding; hindbrain development; transcription, DNA-dependent; stem cell maintenance; liver development; keratinocyte differentiation; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; cytosine methylation within a CG sequence; nucleosome disassembly; methylation-dependent chromatin silencing; ATP-dependent chromatin remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of cell growth; negative regulation of transcription, DNA-dependent Disease: Mental Retardation, Autosomal Dominant 16; Rhabdoid Tumor Predisposition Syndrome 2 |
| NCBI Summary: | The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | P51532 |
| NCBI GenInfo Identifier: | 116242792 |
| NCBI Gene ID: | 6597 |
| NCBI Accession: | P51532.2 |
| UniProt Secondary Accession: | P51532,O95052, Q9HBD3, B1A8Z4, B1A8Z5, B1A8Z6, B1A8Z7 E9PBR8, |
| UniProt Related Accession: | P51532 |
| Molecular Weight: | 1647 |
| NCBI Full Name: | Transcription activator BRG1 |
| NCBI Synonym Full Names: | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 |
| NCBI Official Symbol: | SMARCA4 |
| NCBI Official Synonym Symbols: | BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A |
| NCBI Protein Information: | transcription activator BRG1; SNF2-beta; SNF2-like 4; protein BRG-1; nuclear protein GRB1; brahma protein-like 1; BRM/SWI2-related gene 1; homeotic gene regulator; protein brahma homolog 1; BRG1-associated factor 190A; sucrose nonfermenting-like 4; ATP-dependent helicase SMARCA4; mitotic growth and transcription activator; global transcription activator homologous sequence |
| UniProt Protein Name: | Transcription activator BRG1 |
| UniProt Synonym Protein Names: | ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 |
| Protein Family: | Transcription activator |
| UniProt Gene Name: | SMARCA4 |
| UniProt Entry Name: | SMCA4_HUMAN |
