Description
| 抗体名: | SLC12A5 Antibody (PACO57444) |
| 抗体コード: | PACO57444 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Solute carrier family 12 member 5 protein (944-1071AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO57444 diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO57444 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| バックグラウンド: | Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (By similarity). Involved in the regulation of dendritic spine formation and maturation. |
| シノニム: | Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (hKCC2) (Neuronal K-Cl cotransporter), SLC12A5, KCC2 KIAA1176 |
| UniProt Protein Function: | KCC2: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1. Brain specific. Detected in neuronal cells. Inhibited by WNK3. Belongs to the SLC12A transporter family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: cell soma; integral to plasma membrane; integral to membrane; plasma membrane; dendritic shaft Molecular Function:protein kinase binding; potassium:chloride symporter activity Biological Process: response to drug; synaptic transmission; transport; cellular ion homeostasis; multicellular organism growth; ion transport; learning; transmembrane transport; potassium ion transport; thermosensory behavior |
| NCBI Summary: | K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008] |
| UniProt Code: | Q9H2X9 |
| NCBI GenInfo Identifier: | 161784306 |
| NCBI Gene ID: | 57468 |
| NCBI Accession: | Q9H2X9.3 |
| UniProt Related Accession: | Q9H2X9 |
| Molecular Weight: | |
| NCBI Full Name: | Solute carrier family 12 member 5 |
| NCBI Synonym Full Names: | solute carrier family 12 member 5 |
| NCBI Official Symbol: | SLC12A5 |
| NCBI Official Synonym Symbols: | KCC2; EIG14; hKCC2; EIEE34 |
| NCBI Protein Information: | solute carrier family 12 member 5 |
| UniProt Protein Name: | Solute carrier family 12 member 5 |
| UniProt Synonym Protein Names: | Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; hKCC2; Neuronal K-Cl cotransporter |
| Protein Family: | Solute carrier family |
| UniProt Gene Name: | SLC12A5 |
| UniProt Entry Name: | S12A5_HUMAN |
