Description
| 抗体名: | SEC14L2 Antibody (PACO58893) |
| 抗体コード: | PACO58893 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human SEC14-like protein 2 protein (233-358AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: PC-3 whole cell lysate. All lanes: SEC14L2 antibody at 4.28µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 45, 37 kDa. Observed band size: 47 kDa. |
 | IHC image of PACO58893 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO58893 diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | Carrier protein. Binds to some hydrophobic molecules and promotes their transfer between the different cellular sites. Binds with high affinity to alpha-tocopherol. Also binds with a weaker affinity to other tocopherols and to tocotrienols. May have a transcriptional activatory activity via its association with alpha-tocopherol. Probably recognizes and binds some squalene structure, suggesting that it may regulate cholesterol biosynthesis by increasing the transfer of squalene to a metabolic active pool in the cell. |
| シノニム: | SEC14-like protein 2 (Alpha-tocopherol-associated protein) (TAP) (hTAP) (Squalene transfer protein) (Supernatant protein factor) (SPF), SEC14L2, C22orf6 KIAA1186 KIAA1658 |
| UniProt Protein Function: | SEC14L2: Carrier protein. Binds to some hydrophobic molecules and promotes their transfer between the different cellular sites. Binds with high affinity to alpha-tocopherol. Also binds with a weaker affinity to other tocopherols and to tocotrienols. May have a transcriptional activatory activity via its association with alpha-tocopherol. Probably recognizes and binds some squalene structure, suggesting that it may regulate cholesterol biosynthesis by increasing the transfer of squalene to a metabolic active pool in the cell. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid-binding Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: cytosol; nucleus |
| NCBI Summary: | This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008] |
| UniProt Code: | O76054 |
| NCBI GenInfo Identifier: | 21542232 |
| NCBI Gene ID: | 23541 |
| NCBI Accession: | O76054.1 |
| UniProt Secondary Accession: | O76054,Q53EQ2, Q6PD61, Q9ULN4, B7Z8Q1, F5H3U4, |
| UniProt Related Accession: | O76054 |
| Molecular Weight: | 46kDa |
| NCBI Full Name: | SEC14-like protein 2 |
| NCBI Synonym Full Names: | SEC14 like lipid binding 2 |
| NCBI Official Symbol: | SEC14L2 |
| NCBI Official Synonym Symbols: | SPF; TAP; TAP1; C22orf6 |
| NCBI Protein Information: | SEC14-like protein 2 |
| UniProt Protein Name: | SEC14-like protein 2 |
| UniProt Synonym Protein Names: | Alpha-tocopherol-associated protein; TAP; hTAP; Squalene transfer protein; Supernatant protein factor; SPF |
| Protein Family: | SEC14-like protein |
| UniProt Gene Name: | SEC14L2 |
| Antibodies |
| Anti-SEC14L2 Antibody (CAB10586) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
