Description
| Product Name: | Recombinant Rat sCD86 Protein (FC Tag) |
| SKU: | RPES8517 |
| Size: | 20μg |
| Tag: | C-Fc |
| Reactivity: | Rat |
| Expression Host: | Mammalian |
| Accession: | O35531 |
| Sequence: | Val29-Ile250 |
| Calculated Molecular Weight: | 49.3 kDa |
| Observed Molecular Weight: | 60-80 kDa |
| Synonyms: | Activation B7-2 antigen 3, Activation B7-2 antigen, B-lymphocyte activation antigen B7-2 2, B-lymphocyte activation antigen B7-2, B7 2, B7, B7-2, B7.2, B70, B72 antigen, BU63, CD28 antigen ligand 2 2, CD28 antigen ligand 2, Cd28l2, CD28LG2, CD86, CD86 antigen, CD86 antigen, CD86 molecule, CD86, CLS1, CTLA-4 counter-receptor B7.2 2, 3, CTLA-4 counter-receptor B7.2, Early T-cell costimulatory molecule 1, ETC-1, FUN 1, FUN-1, LAB72, Ly-58, Ly58, MB7, MB7-2, Membrane glycoprotein, MGC34413, T-lymphocyte activation antigen CD86, TS/A-2 |
| Endotoxin: | < 1.0 EU/mg of the protein as determined by the LAL method |
| Purity: | > 90% as determined by reducing SDS-PAGE. |
CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
| Bio-activity: | Not validated for activity |
| Formulation: | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution: | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Storage: | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
