Description
| Product Name: | Recombinant Rat Cystatin-C/CST3 Protein |
| SKU: | RPCB1731 |
| Size: | 10µg |
| Tag: | C-6His |
| Reactivity: | Rat |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Rat Cystatin-C/CST3 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <0.1EU/μg |
| Purity: | > 92% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of 20mM HEPES and 150 mM NaCl，pH 7.0 |
| Gene ID: | 25307 |
Cystatin C, also known as Cystatin-3 (CST3) is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P14841 |
 | Recombinant Rat Cystatin-C/CST3 Protein was determined by SDS- PAGE with Coomassie Blue, showing a band at 18-25 kDa. |
 | Recombinant Rat CST3 inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC . The IC50 value is <3.4 nM, under the described conditions. |
