Description
| Product Name: | Recombinant Mouse IL-1Ra/IL-1F3/IL-1RN Protein |
| SKU: | RPCB0737 |
| Size: | 10µg |
| Tag: | No tag |
| Reactivity: | Mouse |
| Expression Host: | E. coli |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse IL-1Ra/IL-1F3/IL-1RN Protein , tested reactivity inE. coliand has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | > 95% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 16181 |
Interleukin-1 receptor antagonist protein (Il1rn), also known as IL-1ra, IRAP or IL1 inhibitor, is a member of theinterleukin 1 cytokine family. This protein inhibits the activities of interleukin 1 alpha (IL1A) and interleukin 1beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. The mouseIl1rn gene encodes a 178 amino acids (aa) protein with a 26 aa signal peptide. Mouse Il1rn protein shares 26%and 19% identity with its homologues IL-1 beta and IL-1 alpha, respectively. Il1rn can Inhibits the activity ofinterleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP forsignaling, but has no interleukin-1 like activity. Recently, an recombinant human Il1rn protein is used in thetreatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P25085 |
 | Recombinant Mouse IL-1Ra/IL-1F3/IL-1RN Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 15-25 kDa. |
 | Recombinant Mouse IL-1Ra/IL-1F3/IL-1RN inhibit IL-1 alpha-dependent proliferation in D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 2.47-9.86 ng/mL in the presence of 3ng/mL of recombinant human IL-1 alpha, corresponding to a specific activity of 1.01×105~4.05×105 units/mg. |
