Description
| Product Name: | Recombinant Mouse CCL2/MCP-1 Protein |
| SKU: | RPCB1140 |
| Size: | 10µg |
| Tag: | No tag |
| Reactivity: | Mouse |
| Expression Host: | Pichia |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse CCL2/MCP-1 Protein , tested reactivity inPichiaand has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <0.1EU/μg |
| Purity: | > 95% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 20296 |
Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P10148 |
 | Recombinant Mouse CCL2/MCP-1 Protein was determined by SDS- PAGE with Coomassie Blue, showing bands at 14-18 kDa. |
 | Recombinant Mouse CCL2 inhibit the proliferation of HUVEC(Human Umbilical Vein Endothelial Cells). The ED₅₀ for this effect is 0.20-0.80 ng/mL. Recombinant Mouse CCL2 chemoattract THP-1 cells. The ED₅₀ for this effect is typically 2.87-11.48 ng/mL. |
