Description
| Product Name: | Recombinant Mouse Calcitonin/CALCA Protein |
| SKU: | RPCB1780 |
| Size: | 100µg |
| Tag: | C-hFc |
| Reactivity: | Mouse |
| Expression Host: | HEK293 Cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse Calcitonin/CALCA Protein , tested reactivity in HEK293 Cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <1EU/μg |
| Purity: | > 95% as determined by HPLC. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 12310 |
Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | Q99JA0 |
 | Mouse CALCA/CGRP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
 | The purity of Mouse CALCA/CGRP is greater than 95% as determined by SEC-HPLC. |
